Multicomponent analysis with spectrophotometry (mixed colour photometry)

Article no. P3070501 | Type: Experiments

10 Minutes
10 Minutes
University
easy
Teachers/Professors , Students

Principle

In solutions containing different-coloured substances the concentrations of the dyes can be anlysed by spectrometry without prior separation of the substances.
Using the integrated software the spectra of the pure dye solutions and mixtures thereof will be recorded. Calibration curves for each substances enable us to determine the quantity of that substance in the solution.

Benefits

  • Experimental procedure without dangerous chemicals
  • Modern, easy-to-use photospectrometer
  • Simple evaluation by supplied software

Tasks

In a mixed solution containing fuchsine acid and patent blue V, the proporion of fuchsine acid is to be determined.

Learning objectives

  • Photometry
  • UV-VIS spectrometry
  • Lambert-Beer's law
  • Dyes
  • Absorption of light

Necessary accessories

  • Precision balance 620g/0.001g

Software integrated in spectrometer. Computer not provided.

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Digital learning
(en) Versuchsbeschreibung
p3070501_en .pdf
File size 4.69 Mb
pdf
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(ru) Versuchsbeschreibung
p3070501_ru .pdf
File size 4.91 Mb
pdf
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