Technical data

Multicomponent analysis with spectrophotometry (mixed colour photometry)

Article no: P3070501

Principle

In solutions containing different-coloured substances the concentrations of the dyes can be anlysed by spectrometry without prior separation of the substances.
Using the integrated software the spectra of the pure dye solutions and mixtures thereof will be recorded. Calibration curves for each substances enable us to determine the quantity of that substance in the solution.

Benefits

Experimental procedure without dangerous chemicals
Modern, easy-to-use photospectrometer
Simple evaluation by supplied software

Tasks

In a mixed solution containing fuchsine acid and patent blue V, the proporion of fuchsine acid is to be determined.

Learning objectives

Photometry
UV-VIS spectrometry
Lambert-Beer's law
Dyes
Absorption of light

Necessary accessories

Precision balance 620g/0.001g

Software integrated in spectrometer. Computer not provided.

Scope of delivery

Fibre-optics spectrophotometer	35620-00	1
Volumetric flasks, Boro, various sizes	36548-00	9
Graduated pipettes, various sizes	36602-00	1
Microspoon, steel	33393-00	1
Powder funnels, plastic, various sizes	36893-00	1
Patent Blue V (sodium salt), 25 g	48376-04	2
Wash bottle, plastic, 500 ml	33931-00	1
Fuchsine acid -rubin s-, 25 g	31813-04	2
Water, distilled 5 l	31246-81	1
Data cable USB, plug type A/B, 1.8 m	14608-00	1
Macro-cuvettes, PS, 4ml,100 pcs	35663-10	1