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Technical data


Electrophoresis of Lambda DNA

Article no: P8110200

Principle

The most important work methods of molecular genetics are taught in this experiment. With the aid of agarose gel electrophoresis migration velocity and the cleavage pattern of lambda DNA can be investigated.

Benefits

  • Complete equipment set which also contains all required consumables
  • Consumables can be purchased as a replacement kit
  • Ready to use: DNA fragment samples are already predigested
  • Easy to use: detailed experiment guide
  • DNA samples can can be stored for a longer period at room temperature because they are lyophilized

Tasks

  • Cast the agarose gel
  • Prepare samples for electrophoresis
  • Perform electrophoresis
  • Stain the samples and analyse the banding pattern

Learning objectives

  • Lambda DNA
  • Electrophoresis
  • Restriction enzymes
  • Restriction digestion
  • Banding pattern

Scope of delivery

Microliterpipette 2-20 µl, autoclavable 47141-10 1
Microliterpipette 20-200 µl, autoclavable 47141-11 1
Pipette tips, 2-200 µl, 1000 pcs 47148-01 1
Electrophoresis chamber, horizontal KLA-530-200 1
Spatula, steel, l=185mm 46952-00 1
Spoon spatula, nickel-plated, 180 mm 33392-00 1
Protecting glasses, clear glass 39316-00 1
myVolt Mini Electrophoresis power supply 100V/200V 65967-99 1
Hotplate magnetic stirrer with connection for electronic contact thermometer, 3 ltr., 230 V 35760-93 1
Erlenmeyer flasks, Duran®, narrow neck, various sizes 36121-00 1
Graduated cylinders, plastic, various sizes 46288-01 1
Magnetic stirring bar, 50 mm, cylindrical 46299-03 1
Cotton wool, white 200 g 31944-10 1
Water, distilled 5 l 31246-81 1
Rubber gloves, size M (8), one pair 39323-00 1
Kit: Agarose gel electrophoresis of Lambda DNA KLA-530-110 1

PHYWE Systeme GmbH & Co. KG
Robert-Bosch-Breite 10 – 37079 Göttingen – Germany
www.phywe.com