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Technical data


Electrophoresis of Plasmid DNA

Article no: P8110100

Principle

The most important work methods of molecular genetics are taught in this experiment. With the aid of agarose gel electrophoresis migration velocity and the cleavage pattern of plasmid DNA can be investigated.

Vorteile

  • Complete equipment set which also contains all required consumables
  • Consumables can be purchased as a replacement kit
  • Ready to use: DNA fragment samples are already predigested
  • Easy to use: detailed experiment guide
  • DNA samples can can be stored for a longer period at room temperature because they are lyophilized

Aufgaben

  • Cast the agarose gel
  • Prepare samples for electrophoresis
  • Perform electrophoresis
  • Stain the samples and analyse the banding pattern

Learning objectives

  • Plasmid DNA
  • Electrophoresis
  • Restriction enzymes
  • Restriction digestion
  • Banding pattern

 

 

Scope of delivery

Microliterpipette 2-20 µl, autoclavable 47141-10 1
Microliterpipette 20-200 µl, autoclavable 47141-11 1
Pipette tips, 2-200 µl, 1000 pcs 47148-01 1
Electrophoresis chamber, horizontal KLA-530-200 1
Spatula, steel, l=185mm 46952-00 1
Spoon spatula, nickel-plated, 180 mm 33392-00 1
Protecting glasses, clear glass 39316-00 1
myVolt Mini Electrophoresis power supply 100V/200V 65967-99 1
Hotplate magnetic stirrer with connection for electronic contact thermometer, 3 ltr., 230 V 35760-93 1
Erlenmeyer flasks, Duran®, narrow neck, various sizes 36121-00 1
Graduated cylinders, plastic, various sizes 46288-01 1
Magnetic stirring bar, 50 mm, cylindrical 46299-03 1
Cotton wool, white 200 g 31944-10 1
Water, distilled 5 l 31246-81 1
Rubber gloves, size M (8), one pair 39323-00 1
Kit: Bacterial plasmid DNA in gel electrophoresis KLA-530-100 1

PHYWE Systeme GmbH & Co. KG
Robert-Bosch-Breite 10 – 37079 Göttingen – Germany
www.phywe.com