Technical data

Electrophoresis of Plasmid DNA

Article no: P8110100

Principle

The most important work methods of molecular genetics are taught in this experiment. With the aid of agarose gel electrophoresis migration velocity and the cleavage pattern of plasmid DNA can be investigated.

Vorteile

Complete equipment set which also contains all required consumables
Consumables can be purchased as a replacement kit
Ready to use: DNA fragment samples are already predigested
Easy to use: detailed experiment guide
DNA samples can can be stored for a longer period at room temperature because they are lyophilized

Aufgaben

Cast the agarose gel
Prepare samples for electrophoresis
Perform electrophoresis
Stain the samples and analyse the banding pattern

Learning objectives

Plasmid DNA
Electrophoresis
Restriction enzymes
Restriction digestion
Banding pattern

Scope of delivery

Microliterpipette 2-20 µl, autoclavable	47141-10	1
Microliterpipette 20-200 µl, autoclavable	47141-11	1
Pipette tips, 2-200 µl, 1000 pcs	47148-01	1
Electrophoresis chamber, horizontal	KLA-530-200	1
Spatula, steel, l=185mm	46952-00	1
Spoon spatula, nickel-plated, 180 mm	33392-00	1
Protecting glasses, clear glass	39316-00	1
myVolt Mini Electrophoresis power supply 100V/200V	65967-99	1
Hotplate magnetic stirrer with connection for electronic contact thermometer, 3 ltr., 230 V	35760-93	1
Erlenmeyer flasks, Duran®, narrow neck, various sizes	36121-00	1
Graduated cylinders, plastic, various sizes	46288-01	1
Magnetic stirring bar, 50 mm, cylindrical	46299-03	1
Cotton wool, white 200 g	31944-10	1
Water, distilled 5 l	31246-81	1
Rubber gloves, size M (8), one pair	39323-00	1
Kit: Bacterial plasmid DNA in gel electrophoresis	KLA-530-100	1