Kit: Agarose gel electrophoresis of Lambda DNA

Function and Applications

Students map the HindIII and EcoRI restriction sites of phage Lambda using predigested restriction fragments and full length phage. This experiment is also of historical importance because the combination of Lambda phage DNA and the enzymes HindIII/EcoRI mark a milestone in molecular biology that laid the foundations for gene therapy, transgenic organisms and modern biotechnology.

Benefits

• Ready to use: DNA fragment samples are already predigested and prestained
• Easy to use: detailed experiment information
• Little preparation time for teachers
• DNA samples can be pipetted into the gel directly
  

Equipment and Technical Data

Material sufficient for 8 gels (10µl per lane)

The kit contains the following components:

• Lambda DNA (48.5 kb DNA), 120 µl

• Lambda DNA HindIII digest, 120 µl

• Lambda DNA-EcoR1 digest, 120 µl

• Lambda DNA double digest with HindIII and EcoR1, 120 µl

• Detailed user instructions for download

Transport at room temperature, storage under -18°C. Use within 1 year.

Necessary accessories

• TESS advanced Biology set Genetics (15312-88)

When used with the blueGel electrophoresis system 35017-99 (is also included in the Student set Genetics 15312-88), a TBE buffer is also required (e.g. 35018-73).