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Technical data


Paternity test

Article no: P8110801

Principle

This experiment builds a bridge between classical genetics, with its Mendelian rules and the concept of alleles on the one hand, and molecular genetics on the other. It involves the genetic process of paternity analysis as it is used today, namely by means of gel electrophoresis. In this experiment, we help a pack of puppies to find their father.

 

Benefits

  • Complete solution including all the necessary consumables
  • Consumables can be purchased separately as a kit
  • Ready to use: DNA samples can be used directly in electrophoresis without sample preparation
  • Well-documented, simple experimental procedure
  • Band migration can be observed during  electrophoresis

 

Tasks

  • Casting of an agarose gel
  • Sample preparation for gel electrophoresis
  • Conducting gel electrophoresis
  • Analysing the band pattern

 

Learning objectives

  • DNA fingerprinting
  • Paternity test
  • Electrophoresis
  • Banding pattern
  • DNA profile
  • Forensics
  • PCR

 

 

Scope of delivery

Microliterpipette 2-20 µl, autoclavable 47141-10 1
blueGel™ gel - electrophoresis unit with integrated illuminator 35017-99 1
Pipette tips, 2-200 µl, 1000 pcs 47148-01 1
SeeGreen™ Agarose Tabs™, 3-in-1 agarose tablets, for 16 gels 35018-71 1
Graduated pipettes, various sizes 36602-00 1
Protecting glasses, clear glass 39316-00 1
1000 ml, 10x TBE-buffer for gel-electrophoresis, solution 35018-73 1
Erlenmeyer flasks, Boro, wide neck, various sizes 46151-00 1
Graduated cylinders, Boro, various sizes 36629-00 1
Pipettor 36592-00 1
PCR single tubes, 0.2 ml, pack of 100, in bag 35928-01 1
Rack for 8 x 0.2 ml disposable reaction tubes 37652-01 1
Water, distilled 5 l 31246-81 1
Rubber gloves, size M (8), one pair 39323-00 1
Kit: Paternity test of a DNA profile by gel electrophoresis 15312-04 1

PHYWE Systeme GmbH & Co. KG
Robert-Bosch-Breite 10 – 37079 Göttingen – Germany
www.phywe.com