Paternity test Principle This experiment builds a bridge between classical genetics, with its Mendelian rules and the concept of alleles on the one hand, and molecular genetics on the other. It involves the genetic process of paternity analysis as it is used today, namely by means of gel electrophoresis. In this experiment, we help a pack of puppies to find their father. Benefits - Complete solution including all the necessary consumables
- Consumables can be purchased separately as a kit
- Ready to use: DNA samples can be used directly in electrophoresis without sample preparation
- Well-documented, simple experimental procedure
- Band migration can be observed during electrophoresis
Tasks - Casting of an agarose gel
- Sample preparation for gel electrophoresis
- Conducting gel electrophoresis
- Analysing the band pattern
Learning objectives - DNA fingerprinting
- Paternity test
- Electrophoresis
- Banding pattern
- DNA profile
- Forensics
- PCR
Scope of delivery
Microliterpipette 2-20 µl, autoclavable
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47141-10
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1
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blueGel™ gel - electrophoresis unit with integrated illuminator
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35017-99
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1
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Pipette tips, 2-200 µl, 1000 pcs
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47148-01
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1
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SeeGreen™ Agarose Tabs™, 3-in-1 agarose tablets, for 16 gels
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35018-71
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1
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Graduated pipettes, various sizes
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36602-00
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1
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Protecting glasses, clear glass
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39316-00
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1
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1000 ml, 10x TBE-buffer for gel-electrophoresis, solution
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35018-73
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1
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Erlenmeyer flasks, Boro, wide neck, various sizes
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46151-00
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1
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Graduated cylinders, Boro, various sizes
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36629-00
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1
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Pipettor
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36592-00
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1
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PCR single tubes, 0.2 ml, pack of 100, in bag
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35928-01
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1
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Rack for 8 x 0.2 ml disposable reaction tubes
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37652-01
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1
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Water, distilled 5 l
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31246-81
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1
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Rubber gloves, size M (8), one pair
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39323-00
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1
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Kit: Paternity test of a DNA profile by gel electrophoresis
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15312-04
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1
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