Technical data

Electrophoresis of Lambda DNA

Article no: P8110600

Principle

The most important work methods of molecular genetics are taught in this experiment. With the aid of agarose gel electrophoresis migration velocity and the cleavage pattern of lambda DNA can be investigated.

Benefits

Complete equipment set which also contains all required consumables
Consumables can be purchased as a replacement kit
Ready to use: DNA fragment samples are already predigested
Easy to use: detailed experiment guide
DNA samples can can be stored for a longer period at room temperature because they are lyophilized

Tasks

Cast the agarose gel
Prepare samples for electrophoresis
Perform electrophoresis
Stain the samples and analyse the banding pattern

Learning objectives

Lambda DNA
Electrophoresis
Restriction enzymes
Restriction digestion
Banding pattern

Scope of delivery

Microliterpipette 2-20 µl, autoclavable	47141-10	1
blueGel gel - electrophoresis unit with integrated illuminator	35016-99	1
Pipette tips, 2-200 µl, 1000 pcs	47148-01	1
Spoon spatula, nickel-plated, 180 mm	33392-00	1
Protecting glasses, clear glass	39316-00	1
Erlenmeyer flasks, Boro, wide neck, various sizes	46151-00	1
Graduated cylinders, Boro, various sizes	36629-00	1
Storage bottle, clear glass, screw cap, 250 ml	34155-00	1
Graduated cylinders, Boro, various sizes	36630-00	1
Water, distilled 5 l	31246-81	1
500 ml, 10x TBE-buffer for gel-electrophoresis	KLA-530-223	1
Rubber gloves, size M (8), one pair	39323-00	1
Graduated pipettes, various sizes	36602-00	1
Kit: Agarose gel electrophoresis of Lambda DNA	KLA-530-110	1
Pipettor	36592-00	1
SeeGreen™ Agarose Tabs™, 3-in-1 agarose tablets, for 16 gels	35018-71	1