Technical data

Reaction kinetics with spectrophotometry

Article no: P3070601

Principle

The organic dye crystal violet is decolorised in an alkaline medium. This reaction takes place according to a velocity-time law, hence it is a reaction of the first order.
Using the integrated software, the change in concentration of the dye can be traced by measuring the extinction at the wavelength of the absorption maximum. The computer program plots the kinetic data in graphical form.

Benefits

Modern, easy-to-use photospectrometer
Simple evaluation by integrated software

Tasks

The order of reaction during decolorisation of crystal violet is to be determined.

Learning objectives

Reaction kinetics
First order reaction
Photometry
Reaction rate

Necessary accessories

Precision balance 620g/0.001g

Scope of delivery

Fibre-optics spectrophotometer	35620-00	1
Volumetric flasks, Boro, various sizes	36548-00	2
Bulb pipettes, various sizes	36578-00	2
Pipettor	36592-00	1
Beakers, Boro, high form, various sizes	46026-00	3
Microspoon, steel	33393-00	1
Powder funnels, plastic, various sizes	36893-00	1
Wash bottle, plastic, 500 ml	33931-00	1
Sodium hydroxide, pellets, 500 g	30157-50	1
Water, distilled 5 l	31246-81	1
Crystal violet f.bacteriology,25g	31488-04	1
Data cable USB, plug type A/B, 1.8 m	14608-00	1
Macro-cuvettes, PS, 4ml,100 pcs	35663-10	1