Reaction kinetics with spectrophotometry Principle The organic dye crystal violet is decolorised in an alkaline medium. This reaction takes place according to a velocity-time law, hence it is a reaction of the first order.
Using the integrated software, the change in concentration of the dye can be traced by measuring the extinction at the wavelength of the absorption maximum. The computer program plots the kinetic data in graphical form. Benefits - Modern, easy-to-use photospectrometer
- Simple evaluation by integrated software
Tasks The order of reaction during decolorisation of crystal violet is to be determined. Learning objectives - Reaction kinetics
- First order reaction
- Photometry
- Reaction rate
Necessary accessories - Precision balance 620g/0.001g
Scope of delivery |
Fibre-optics spectrophotometer
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35620-00
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1
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Volumetric flasks, Boro, various sizes
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36548-00
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2
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Bulb pipettes, various sizes
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36578-00
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2
| |
Pipettor
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36592-00
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1
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Beakers, Boro, high form, various sizes
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46026-00
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3
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Microspoon, steel
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33393-00
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1
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Powder funnels, plastic, various sizes
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36893-00
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1
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Wash bottle, plastic, 500 ml
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33931-00
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1
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Sodium hydroxide, pellets, 500 g
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30157-50
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1
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Water, distilled 5 l
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31246-81
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1
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Crystal violet f.bacteriology,25g
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31488-04
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1
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Data cable USB, plug type A/B, 1.8 m
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14608-00
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1
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Macro-cuvettes, PS, 4ml,100 pcs
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35663-10
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1
| |
Analytical Balance, Sartorius, 220 g : 0.1 mg
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49300-99
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1
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