Polarimetry Principle The rotation of the plane of polarisation through a sugar solution measured with a half-shade polarimeter and the reaction rate constant for the inversion of cane sugar determined. Benefits - Even transparent fluids can change the properties of light passing them
- Find out how fast one type of sugar changes into another one
- Use optical methods, involving the plane of polarisation, to observe chemical processes
Tasks - To determine the specific rotation of cane sugar (sucrose) and lactose by measuring the rotation of various solutions of known concentration.
- To determine the reaction rate constant when cane sugar is transformed into invert sugar.
Learning objectives - Half-shade principle
- Optical rotatory power
- Optical activity
- Saccharimetry
- Specific rotation
- Reaction rate
- Weber-Fechner law
Scope of delivery
Polarimeter, LED, 590 nm
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35907-99
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1
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Immersion thermostat Alpha A, 230 V
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08493-93
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1
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External circulation set for thermostat Alpha A
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08493-02
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1
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Bath for thermostat, makrolon
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08487-02
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1
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Digital stopwatch, 24 h, 1/100 s and 1 s
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24025-00
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1
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Crucible tongs, 200 mm, stainless steel
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33600-00
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1
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Beaker, 250 ml, plastic (PP)
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36013-01
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2
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Graduated cylinders, plastic, various sizes
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36629-01
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2
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Graduated beaker, 1000 ml, plastic (PP)
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36640-00
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1
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Funnels, plastic, various sizes
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36891-00
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1
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Spoon, with spatula end, 180 mm, plastic
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38833-00
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1
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Glass stirring rod, d = 8 mm, l = approx. 300 mm
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40485-06
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1
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Pipette with rubber bulb, long
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64821-00
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1
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D (+)-Sucrose 100 g
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30210-10
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1
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Hydrochloric acid 37 %, 1000 ml
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30214-70
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1
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Water, distilled 5 l
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31246-81
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1
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D(+)-Lactose, powder 100 g
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31577-10
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1
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Balance OHAUS LG 311, 4 beams, 0...311 g
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44007-31
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1
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Tubing connector, ID 6-10mm
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47516-01
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2
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