Detection of quinine in tonic water

Principle

Chinine is the main alkaloid of the bark of the Chinabaum. It has a typical, bitter taste and is hardly soluble in water, but soluble in alcohol, ether and chloroform

In this experiment, chinine is extracted from tonic water with an organic solvent and detected by its fluorescence under UV light.

Learning objectives

Seperation of chinine from liquid mixtures
Detection of chinine
fluorescence under UV light

Device name

Article no.

Quantity

UV analysis lamp 254/365 nm

Article no. 33972-93

1

Support base, variable

Article no. 02001-00

1

Stand rods, stainless steel, various sizes | Length: 600 mm

Article no. 02037-00

4

Boss head

Article no. 02043-00

4

Universal clamp

Article no. 37715-01

2

Support ring, i.d. 70mm,

Article no. 37721-01

1

Heating mantle f. roundbottom flask, 100 ml

Article no. 49541-93

1

Clamp for heating mantle

Article no. 49557-01

1

Round bottom flask w. side arm, 100 ml, PN19

Article no. 34885-00

1

Erlenmeyer flasks, Duran®, wide neck, various sizes | Volume: 100 ml

Article no. 36128-00

1

Cooling jacket, GL 25/8

Article no. MAU-27225000

1

Rubber stopper, d = 22/17 mm, 1 hole

Article no. 39255-01

1

Beakers, Boro, low form, various sizes | Volume: 250 ml

Article no. 46054-00

1

Beakers, Boro, high form, various sizes | Volume: 250 ml

Article no. 46027-00

2

Graduated cylinders, Boro, various sizes | Volume: 100 ml

Article no. 36629-00

1

Graduated pipettes, various sizes | Volume: 10 ml

Article no. 36600-00

2

Separatory funnel 250 ml pear-sh.

Article no. 36884-00

1

Precision Balance, Sartorius, 620 g : 1 mg

Article no. 49311-99

1

Rubber hose, various diameters | diameter: 6 mm

Article no. 39282-00

2

Hose clip, diam. 8-16 mm, 1 pc.

Article no. 40996-02

2

Students thermometer,-10...+110°C, l = 180 mm

Article no. 38005-02

1

Boiling beads, 200 g

Article no. 36937-20

1

Laboratory pen, waterproof, black

Article no. 38711-00

1

Test tube, laboratory glass, various lengths, 100 pcs. | Length: 180 mm

Article no. 37658-10

1

Test tube rack, wood, for 6 tubes d= 22mm

Article no. MAU-20042200

1

Test tube holder, up to d 22mm

Article no. 38823-00

1

Pipette with rubber bulb

Article no. 64701-00

4

Pipettor

Article no. 36592-00

1

Spoon, special steel

Article no. 33398-00

1

Protecting glasses, clear glass

Article no. 39316-00

1

Rubber gloves, size M (8), one pair

Article no. 39323-00

1

Sodium carbonate, anhyd. 250 g

Article no. 30154-25

1

Sodium chloride, 500 g

Article no. 30155-50

1

Petroleum ether, 40-60 C 250 ml

Article no. 30184-25

1

Sulphuric acid, 95-97%, 500 ml

Article no. 30219-50

1

Glycerol, 250 ml

Article no. 30084-25

1

Water, distilled 5 l

Article no. 31246-81

1

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Detection of quinine in tonic water

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