Electrophoresis of Plasmid DNA

Principle

The most important work methods of molecular genetics are taught in this experiment. With the aid of agarose gel electrophoresis migration velocity and the cleavage pattern of plasmid DNA can be investigated.

Benefits

• Complete equipment set which also contains all required consumables
• Consumables can be purchased as a replacement kit
• Ready to use: DNA fragment samples are already predigested
• Easy to use: detailed experiment guide
• DNA samples can can be stored for a longer period at room temperature because they are lyophilized

Tasks

1. Casting agarose gels
2. Preparing samples for electrophoresis
3. Performing electrophoresis
4. Staining the samples and analyse the banding pattern

Learning objectives

• Plasmid DNA
• Electrophoresis
• Restriction enzymes
• Restriction digestion
• Banding pattern